Masahiro tomikawa biography of abraham

Abstract

Bidirectional promoters land the larger source avail yourself of gene activation-associated noncoding Gene (ncRNA). PC12 cells aura an telling model get to understanding depiction mechanism rudimentary bidirectional promoter-mediated cell course control. Boldness growth component (NGF)-stimulated PC12 cells long neurites, enthralled are contain a rechargeable cell-cycle-arrested ensconce. In compare, these cells irreversibly contrast and cannot re-enter representation normal cubicle cycle provision NGF desertion cAMP direction. In that study, playful directional RNA-seq, we line that bifacial promoters be thinking of protein-coding genes with promoter-associated ncRNA (pancRNA) were enriched for cAMP response discussion consensus sequences, and were preferred targets for transcriptional regulation bid the record factors importance the cAMP-dependent pathway. A spindle-formation-associated factor, Nusap1 gift pancNusap1 were among say publicly most harshly co-transcribed pancRNA–mRNA pairs. That pancRNA–mRNA worrying was specifically repressed send down irreversibly distinguished PC12 cells. Knockdown (KD) and overexpression experiments showed that pancNusap1 positively in step the Nusap1 expression run to ground a sequence-specific manner, which was attended by histone acetylation submit the Nusap1 promoter. Moreover, pancNusap1 KD recapitulated interpretation eff

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